Biodiversity characters of the landscape provide basis of prioritizing the sites in conservation effort. There is an urgent need for rapid assessment of existing biodiversity using state-of-art tools and technologies at large scale. The purpose of the study is to model and prioritize biological richness based on multi-criteria decision analysis (MCDA) for conservation priority and management planning. Vegetation type map for year 2017 was developed for generation of various landscape indices e.g. fragmentation, patchiness, porosity, juxtaposition etc. The Spatial Biodiversity Model (SBM) prepared for similar landscape of Uttarakhanda, India which is scale, resolution and location independent for spatial biodiversity richness modelling was executed in R programming platform. Satellite data, non-spatial data and ancillary data were used to generate Biological Richness (BR) map which is categorized into 4 classes as low, moderate, high and very high (biodiversity rich) including non-forest area to quantify BR area. The result shows that largest area is under very high biological richness class followed by high, moderate and low BR area. Overall accuracy and Kappa Statistics of LULC/vegetation type classification is 82.61% and 0.8013 respectively. The spatial regression analysis for final output validation has been made with ground based species diversity data where R² value for Shannon-Wiener index and Margalef’s diversity index are 0.64 and 0.56 respectively. The results also re-emphasize the role of geospatial techniques in the quick appraisal of predicting biological richness. The study result is applicable in systematic inventory of biological resources, land use planning, conservation prioritization and policy support.