Hydrodynamic tunnel has proved to be an effective mean for studying aerodynamic processes using scaled models of real aircrafts. The specificity of study in a hydrodynamic tunnel requires to apply non-contact measuring techniques, such as photogrammetry, for retrieving information on geometrical characteristics of the flow. So reliable calibration of photogrammetric system is a key element for trustworthy analysis of flow behaviour. For 3D measurements in a hydrodynamic tunnel calibration technique must account for refraction effects at optical media interfaces. But often the design of a hydrodynamic tunnel does not allow to perform standard calibration procedure based on image acquisition of a special test field, placed in the working space of the measuring system. The presented study addresses this problem – developing the technique for accurate photogrammetric calibration, for the case of the working space being inaccessible for placing there a test field. The developed calibration technique estimates parameters of interior orientation of the photogrammetric system and parameters of multimedia optical environment by special preliminary procedure, that allows to obtain accurate 3D measurements during experiments in hydrodynamic tunnel. Experimental evaluation of the developed technique demonstrated high accuracy of photogrammetric 3D measurements.