Recent advances in remote sensing technologies have allowed the development of new innovative methodologies to obtain geospatial information about Eucalyptus genus distribution. This is an important task for forest stakeholders due to the high presence of this genus in forest plantations worldwide. Therefore, the next step in research should focus on exploring remote sensing possibilities to discern between Eucalytpus species. It would be an important step forward in forest management since different Eucalyptus species present different characteristics and properties that imply different management plans and industrial usages. This study accomplish the classification of <i>E. nitens</i> and <i>E. globulus</i>, the most common Eucalyptus species in the Iberian Peninsula. Worldview-3 images and random forest are used in a forest area placed in Galicia (Northwest of Spain). The differentiation of Eucalyptus species resulted in a producer’s accuracy of 84% and a users’ accuracy of 70% for <i>E. nitens</i>, while for <i>E. globulus</i> accuracy metrics did not reach 70%. The most important bands in the classification were the coastal blue and the blue, followed by the red related ones. The resulting unequal accuracy metrics might be caused by an imbalanced presence of both species in the selected study area. Therefore, further studies might be developed in different locations.