Time series (TS) analysis has always been an important topic in Remote Sensing (RS). Open access to archives of Earth Observation missions, periodically acquired nation-wide aerial imagery and LiDAR point clouds, and various research datasets, together with open tools for TS data processing, have brought new possibilities in RS research but also challenges in education. The topic of TS analysis in RS has become an essential part of MSc curricula in geoinformatics, geography, and related fields. Open learning materials systematically addressing the issue of TS at the master's level are rare and their development is time and resource demanding. Thus, based on previous collaboration, the four research groups from Charles University, Heidelberg University, University of Innsbruck, and University of Warsaw joint their specific expertise and developed an open E-learning course on Time Series Analysis in RS for Understanding Human-Environment Interactions (E-TRAINEE). The course consists of four Modules covering the topics of TS from general approaches (M1) to specific methods of processing TS of satellite multispectral images (M2), 3D/4D point clouds (M3), and aerial image and laboratory spectroscopy (M4). Theoretical parts are supported with exercises/tutorials and case studies based on research activities of the involved teams. The course is accessible via a web site and is published under the CC-BY SA 4.0 license. The primary target group are MSc and PhD students of geoinformatics and geography, but it is also relevant to students of environmental studies, ecology, or geology, as well as potential users from the public and private sectors dealing with applications of RS.