Ecosystem respiration is influenced by daytime (DT) and night-time (NT) phenomenon. We studied the potential impacts of experimental warming on DT and NT respiration rates. Warming increased both DT and NT respiration rates. Q10 analyses revealed that NT respiration rates are more sensitive to warming than DT. This is the first study addressing the gap of monitoring night-time respiration along with daytime to estimate the respiration rates under experimental warming.